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目的探讨体外受精后不同时期观察受精对胚胎质量的影响。方法随机对进行体外受精-胚胎移植(in vitro fertilization-embryo transfer,IVF-ET)治疗的264个周期分两组进行观察,均于取卵后2～6小时授精。A组为实验组(176周期):于受精后5小时左右去除卵丘细胞提早进行受精观察;B组为对照组(88周期):受精后20小时左右去除卵丘细胞观察原核形成的情况。分别统计两组的年龄、不孕年限、获卵数、正常受精率、异常受精率、优质胚胎率。结果两组的女方年龄、不孕年限、获卵数、正常受精率、异常受精率、优质胚胎率等差异无统计学意义(P>0.05)。结论IVF-ET中提早观察受精对胚胎质量无明显影响。
Objective To investigate the effects of different stages of in vitro fertilization on embryo quality after in vitro fertilization. Methods 264 cycles of in vitro fertilization-embryo transfer (IVF-ET) were randomly divided into two groups. All were fertilized 2 to 6 hours after ovulation. Group A was the experimental group (176 cycles). The cumulus cells were removed about 5 hours after fertilization and early fertilization observation was performed. Group B was the control group (88 cycles): cumulus cells were removed about 20 hours after fertilization to observe the formation of pronucleus. The age, duration of infertility, number of oocytes retrieved, rate of normal fertilization, rate of abnormal fertilization and rate of high-quality embryos were calculated respectively. Results There was no significant difference in female age, infertility duration, number of oocytes retrieved, normal fertilization rate, abnormal fertilization rate and good embryo rate between the two groups (P> 0.05). Conclusion Early observation of fertilization in IVF-ET has no significant effect on embryo quality.