论文部分内容阅读
目的:观察京尼平苷对MPTP帕金森病小鼠黑质多巴胺能神经元的保护作用和对帕金森病的治疗作用。方法:制作MPTP帕金森病小鼠模型,将C57BL/6小鼠随机分为四组:对照组、京尼平苷组、MPTP组、和治疗组(MPTP+Geniposide)。通过行为学实验(转棒实验、游泳试验)、TH免疫组化实验和TUNEL染色,观察京尼平苷对MPTP小鼠行为学、黑质多巴胺能神经元数目和凋亡细胞数目的影响。结果:MPTP组出现了典型的PD行为学改变,小鼠掉落潜伏期和游泳行为评分均低于对照组(P<0.01),黑质致密部TH阳性细胞数目较对照组明显减少(P<0.001),凋亡细胞数目明显增多(P<0.001)。治疗组与MPTP组相比,能明显改善MPTP诱导的C57BL/6小鼠的行为学异常,小鼠掉落潜伏期明显延长(P<0.01),游泳行为评分亦显著提高(P<0.01),同时,TH阳性细胞数目明显增多(P<0.001),凋亡细胞数目明显减少(P<0.001)。结论:京尼平苷能够明显改善MPTP引起的小鼠行为学改变,并能够抑制小鼠黑质TH阳性神经元的减少和凋亡细胞的增加。提示京尼平苷对MPTP帕金森病小鼠DA能神经元具有神经保护效应,这种保护作用可能与京尼平苷的抗凋亡作用有关。
Objective: To observe the protective effect of geniposide on substantia nigra dopaminergic neurons in mice with Parkinson’s disease (MPTP) and its therapeutic effect on Parkinson’s disease. Methods: Mice model of Parkinson disease (MPTP) was made. C57BL / 6 mice were randomly divided into four groups: control group, geniposide group, MPTP group, and treatment group (MPTP + Geniposide). The effects of geniposide on behavior, number of substantia nigra dopaminergic neurons and number of apoptotic cells in MPTP mice were observed by behavioral tests (rod test, swimming test), TH immunohistochemistry and TUNEL staining. Results: The typical behavioral changes of PD in MPTP group were observed. The dropping latency and swimming behavior score of mice in MPTP group were lower than those in control group (P <0.01), and the number of TH positive cells in substantia nigra compact zone was significantly lower than that in control group (P <0.001) ), The number of apoptotic cells increased significantly (P <0.001). Compared with the MPTP group, the treatment group could significantly improve the behavioral abnormalities induced by MPTP in C57BL / 6 mice, and the latency of the mice in the treatment group was significantly longer (P <0.01) and the swimming behavior score was significantly higher (P <0.01) , The number of TH positive cells increased significantly (P <0.001), and the number of apoptotic cells decreased significantly (P <0.001). Conclusion: Geniposide can significantly improve the behavioral changes in mice induced by MPTP, and can inhibit the decrease of TH positive neurons in substantia nigra and the increase of apoptotic cells in mice. These results suggest that geniposide has a neuroprotective effect on DA neurons in mice with MPTP. This protective effect may be related to anti-apoptotic effects of geniposide.