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目的比较HPLC和微生物法测定万古霉素血药浓度的差异。方法30名病人静脉滴注万古霉素1.0g,1次·d-1,分别用HPLC和微生物法测定用药前后的血药浓度。结果HPLC法在5.0～80μg·mL-1浓度内线性关系良好,回收率97.3%～101.6%,日内、日间RSD分别为1.74%～4.06%和1.22%～4.21%;微生物法线性范围在5.0～60μg·mL-1,回收率:98.2%～100.8%,日内、日间RSD分别为4.22%～5.51%和4.59%～6.49%。结论2种测定方法回收率和精密度均符合要求,测得的血药浓度无显著性差异。
Objective To compare the difference of vancomycin blood concentration between HPLC and microbiology. Methods Thirty patients were intravenously infused with 1.0g vancomycin once a day, and the blood levels were determined by HPLC and microbiological methods before and after treatment. Results The HPLC method showed a good linearity in the range of 5.0 ~ 80μg · mL-1 with the recoveries of 97.3% ~ 101.6%. The intra- and inter-day RSD were 1.74% -4.06% and 1.22% -4.21%, respectively. The linear range of microbial method was 5.0 ~ 60μg · mL-1, the recovery rate was 98.2% ~ 100.8%. The intra-day and inter-day RSD were 4.22% ~ 5.51% and 4.59% ~ 6.49%, respectively. Conclusion The recovery and precision of the two methods are in line with the requirements, the measured plasma concentration was no significant difference.