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目的研究铁离子在大鼠实验性慢性脑积水发生中的作用。方法SD雌性大鼠75只,采用随机数字表法分为3组:铁离子组、半量铁离子组及人工脑脊液组。分别将浓度为6.67、3.33g/L的三氯化铁溶液及人工脑脊液各5μl注入大鼠右侧侧脑室,于术后1、14d及28d处死大鼠,评价脑积水发生情况并测量侧脑室侧室指数(侧脑室面积/脑片面积×100),观察脑组织病理变化。结果术后28d(n=10)铁离子组9只大鼠发生脑积水,半量铁离子组(n=5)1只大鼠发生脑积水,两组相比差异显著(P<0.01),人工脑脊液组(n=10)未出现脑积水;铁离子组侧室指数(6.87±2.19)大于半量铁离子组(3.32±0.95)及人工脑脊液组(1.09±0.45)(P<0.01)。铁离子组室周脑组织细胞增生,出现大量泡沫状细胞,脉络丛水肿,含铁血黄素的脑室壁沉积,后期出现脉络丛萎缩,室管膜细胞数量减少,纤毛脱落;半量铁离子组相对较轻;人工脑脊液组无明显变化。结论侧脑室注入高浓度铁离子可诱发脑积水。
Objective To study the role of iron ion in the experimental chronic hydrocephalus in rats. Methods Seventy-five female SD rats were randomly divided into three groups: iron group, half-iron group and artificial cerebrospinal fluid group. Respectively, the concentration of 6.67,3.33g / L of ferric chloride solution and artificial cerebrospinal fluid each 5μl into the right lateral ventricle in rats after 1,14 d and 28 d after the rats were sacrificed to evaluate the occurrence of hydrocephalus and measure the side Ventricular lateral ventricle index (lateral ventricular area / slice area × 100), pathological changes observed. Results Hydrocephalus occurred in 9 rats in Fe + Fe group (n = 10) 28 days after operation (n = 5). There was significant difference between the two groups (P <0.01) (N = 10) did not show hydrocephalus. The side index (6.87 ± 2.19) in ferrous ion group was higher than that in ferric ion group (3.32 ± 0.95) and artificial cerebrospinal fluid group (1.09 ± 0.45) (P <0.01). In the Fe 2+ group, a large amount of foamy cells, choroid plexus edema and deposition of hemosiderin were found in the pericardium of rats in the Fe group. Choroid plexus atrophy appeared later, the number of ependymal cells decreased and the cilia shed. Lighter; no significant change in artificial cerebrospinal fluid group. Conclusion Intracerebroventricular injection of high concentrations of iron ions can induce hydrocephalus.