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植物名称:茶梅(Camellia sasanqua)品种为“笑颜”、“绯乙女”等。材料类别:多年生品种的当年生嫩茎或芽。培养条件:以修改的MS为基本培养基,每升附加BA 1—4 mg,NAA 0.05—0.2 mg,生物素0.01mg。培养室温度白天为27℃,夜间20℃左右;每日光照11小时,光照度为1000lx。生长与分化情况:切取0.6cm左右长的嫩茎节,接种于上述培养基中,45天左右腋芽生长。以芽为外植体接种于上述培养基中,经一个月,培养物基部产生丛生芽,分刈并转移于新鲜培养基中,经二个月每个外植体平均产生6—12个不定芽,其
Plant Name: Camellia sasanqua varieties of “smile”, “Fei Girls” and so on. Material Type: Perennial varieties of perennial shoots or buds. Culture conditions: The modified MS as the basic medium, each additional BA 1-4 mg, NAA 0.05-0.2 mg, biotin 0.01mg. Cultivation room temperature day 27 ℃, night 20 ℃; daily light 11 hours, illumination 1000lx. Growth and differentiation: cut about 0.6cm long tender stem section, inoculated in the above medium, about 45 days axillary bud growth. Buds as explants inoculated in the above medium, after a month, the base of the culture produce clusters of buds, mowing and transfer in fresh medium, each explant after two months an average of 6-12 uncertainties Buds, its