论文部分内容阅读
目的探讨胰岛素样生长因子结合蛋白2(IGFBP-2)在脂肪肝组织中的表达及意义。方法 60只清洁级雄性SD大鼠随机均分为6组:模型组分别用四氯化碳(CCl4)、酒精、高脂饮食诱导,相应的对照组予以正常饮食。采用半定量免疫组织化学方法检测脂肪肝大鼠肝脏组织的IGFBP-2表达。结果三个脂肪肝模型组大鼠肝脏组织IGFBP-2表达均高于各自的对照组[(245.3±67.6)个/mm2vs.(53.5±15.3)个/mm2,(282.4±58.6)个/mm2vs.(61.2±14.2)个/mm2,(352.8±81.3)个/mm2vs.(66.2±13.7)个/mm2](P<0.01),且高脂模型组大鼠肝脏组织IGFBP-2表达高于CCl4模型组和酒精模型组大鼠(P<0.05)。结论 IGFBP-2可能参与脂肪肝发生发展,尤其与非酒精性脂肪肝的形成具有密切联系。
Objective To investigate the expression of insulin-like growth factor binding protein 2 (IGFBP-2) in fatty liver and its significance. Methods Sixty clean male Sprague-Dawley rats were randomly divided into 6 groups: model group were induced by carbon tetrachloride (CCl4), alcohol and high fat diet, and the corresponding control group were given normal diet. Semi-quantitative immunohistochemistry was used to detect the expression of IGFBP-2 in the liver of rats with fatty liver. Results The expression of IGFBP-2 in the liver tissue of three fatty liver model rats were higher than that of the control group [(245.3 ± 67.6) /mm2vs.(53.5 ± 15.3) /mm2, (282.4 ± 58.6) / mm2vs. (61.2 ± 14.2) /mm2, (352.8 ± 81.3) /mm2vs.(66.2 ± 13.7) /mm2](P<0.01), and the expression of IGFBP-2 in the liver tissue of high-fat model group was higher than that of CCl4 model Group and alcohol model rats (P <0.05). Conclusion IGFBP-2 may be involved in the development of fatty liver, especially with the formation of non-alcoholic fatty liver.