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目的探讨当归对阴虚哮喘BALB/c小鼠Th2细胞优势免疫应答的影响。方法采用注射卵清白蛋白(ovalbumin,OVA)致敏、吸入OVA激发的方法来复制BALB/c小鼠哮喘模型,实验后期给予甲状腺素以复制阴虚哮喘模型,通过观测哮喘行为学、血清及支气管肺泡灌洗液(broncho-alveolar lavage fluid,BALF)中IL-4、IL-13的水平、BALF及肺组织中炎性细胞的含量、肺组织中GATA-3蛋白表达水平,探讨当归的平喘作用及对Th2细胞优势免疫应答的影响。结果当归可明显减少阴虚哮喘小鼠哮喘发作的次数及其发作时的症状,改善肺组织病理学而减少肺组织及BALF中炎性细胞的数量,降低血清及BALF中IL-4、IL-13的水平,抑制肺组织中GATA-3蛋白的表达(P<0.05或0.01);同时,当归与地塞米松配伍后对嗜酸粒细胞、IL-4及GATA-3的抑制作用具有一定的协同作用(P<0.05或0.01)。结论当归具有平喘作用,抑制细胞因子IL-4、IL-13及转录因子GATA-3的表达而缓解Th2优势免疫应答可能是其作用机制之一。
Objective To investigate the effect of Angelica sinensis on the predominant immune response of Th2 cells in asthmatic BALB / c mice with asthenospermia. Methods The BALB / c mouse model of asthma was induced by ovalbumin (OVA) sensitization and inhalation of OVA challenge. Thyroxine was administered to the asthmatic model of asthenospermia at the end of the experiment. Asthmatic behavior, serum and bronchus The level of IL-4 and IL-13 in bronchoalveolar lavage fluid (BALF), the content of inflammatory cells in BALF and lung tissue, the expression of GATA-3 in lung tissue and the level of IL-4 and IL- Effect and its influence on the predominant immune response of Th2 cells. Results Angelica can significantly reduce the number of asthma attacks in asthma and the symptoms at onset, improve lung histopathology and reduce the number of inflammatory cells in the lung tissue and BALF, reduce serum and BALF IL-4, IL- 13, and inhibited the expression of GATA-3 protein in lung tissue (P <0.05 or 0.01). Meanwhile, the inhibitory effect of Angelica sinensis and dexamethasone on eosinophils, IL-4 and GATA-3 had certain Synergy (P <0.05 or 0.01). Conclusion Angelica can relieve the predominant Th2 immune response by inhibiting asthma, inhibiting the expression of IL-4, IL-13 and GATA-3.