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目的:探讨不同血清学方法对小儿肺炎支原体(MP)感染患者检测结果的影响及其临床应用。方法:选取2014年5月—2015年6月间收治的MP感染患儿112例基本资料,采用回顾性分析法,分析采用酶联免疫法和冷凝集试验法检测的肺炎支原体抗体的检测结果,并比较不同检测方法对诊断小儿MP感染的价值。结果:112例患儿ELISA检测阳性率为95.54%;冷凝集试验检测阳性率为84.82%;ELISA检测阳性率高于冷凝集试验法(P<0.05);ELISA法检测男性患儿MP感染的阳性率为95.38%,检测女性患儿感染阳性率为95.74%,经组间比较其差异无统计学意义(P>0.05);冷凝集试验法检测男性患儿MP感染的阳性率为84.62%,检测女性患儿感染阳性率为85.12%,经组间比较其差异无统计学意义(P>0.05)。结论:采用ELISA法检测小儿MP感染的阳性率高于冷凝集试验法,临床应针对患儿自身情况选择最佳检测方法,以避免漏检而发生误诊。
Objective: To investigate the effect of different serological methods on the detection of Mycoplasma pneumoniae (MP) infection in children and its clinical application. Methods: The basic data of 112 children with MP infection admitted to our hospital from May 2014 to June 2015 were retrospectively analyzed. The detection results of mycoplasma pneumoniae antibody by enzyme-linked immunosorbent assay (ELISA) and cold agglutination test And compare the value of different detection methods to diagnose MP infection in children. Results: The positive rate of ELISA was 95.54% in 112 children, 84.82% in cold agglutination test, the positive rate of ELISA test was higher than that of cold agglutination test (P <0.05), and the positive rate of MP infection in male children was detected by ELISA The rate of positive rate was 95.38%. The positive rate of detection of female infection in children was 95.74%. There was no significant difference between the two groups (P> 0.05). The positive rate of MP infection in male children was 84.62% by cold agglutination test. The positive rate of female infection in children was 85.12%. There was no significant difference between the two groups (P> 0.05). Conclusion: The positive rate of MP infection in children by ELISA is higher than that of cold agglutination test, and the best detection method should be selected according to the situation of children in order to avoid misdiagnosis.