论文部分内容阅读
目的:检测白介素-3受体α亚基(IL-3Rα)在急性髓细胞性白血病(acute myeloid leukemia,AML)中的表达,研究其在AML发病和预后评估中的意义。方法:应用逆转录-聚合酶链反应(RT-PCR)法检测57例AML患者IL-3Rα的表达,其中包括52例初治和复发患者(6例M1、28例M2、10例M3、3例M4、5例M5)及5例缓解患者,另10例正常人作为对照。结果:①10例正常人和5例AML缓解患者未检测到IL-3Rα表达。②52例初治和复发的AML患者中IL-3Rα阳性表达21例,占40.38%,M1～M5各型患者的阳性率差异无显著性(P>0.05)。初治和复发AML患者之间IL-3Rα阳性表达无明显差异(P>0.05)。③AML患者的IL-3Rα阳性表达与外周血白细胞计数、骨髓中原始细胞比例、CD34阳性表达有关(P<0.05)。④AML患者中IL-3Rα阳性表达的患者CR率低于阴性者(P<0.05)。结论:①IL-3Rα在AML发病中有一定作用。②IL-3Rα基因可作为AML预后不良的一个指标。
Objective: To detect the expression of interleukin-3 receptor α subunit (IL-3Rα) in acute myeloid leukemia (AML) and to study its significance in the pathogenesis and prognosis evaluation of AML. Methods: The expression of IL-3Rα in 57 AML patients was detected by reverse transcription-polymerase chain reaction (RT-PCR), including 52 untreated and relapsed patients (6 patients with M1, 28 patients with M2, 10 patients with M3, Cases of M4, 5 cases of M5) and 5 cases of remission patients, the other 10 cases of normal people as a control. Results: ① IL-3Rα expression was not detected in 10 normal subjects and 5 AML patients. ② The positive expression rate of IL-3Rα in 52 newly diagnosed and relapsed AML patients was 21 (40.38%). There was no significant difference in the positive rate of M1-M5 between the two groups (P> 0.05). There was no significant difference in IL-3Rα expression between newly diagnosed and relapsed AML patients (P> 0.05). (3) The positive expression of IL-3Rα in AML patients was correlated with the count of peripheral blood leukocytes, the percentage of blast cells in bone marrow and the positive expression of CD34 (P <0.05). ④ The CR rate of IL-3Rα-positive patients in AML patients was lower than that in negative patients (P <0.05). Conclusion: ①IL-3Rα plays a role in the pathogenesis of AML. ② IL-3Rα gene can be used as an indicator of poor prognosis of AML.