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目的:测定苦豆子提取物(AL)小鼠灌胃给药的半数致死量,并初步考察其对实验性肝损伤的影响。方法:采用改良寇式法计算半数致死量及95%可信限;以D-氨基半乳糖(GaLN)腹腔注射建立化学性肝损伤模型,以卡介苗加脂多糖小鼠尾静脉注射建立免疫性肝损伤模型,检测小鼠血清转氨酶水平并取其肝脏做组织病理学检查。结果:AL小鼠灌胃给药1次的LD50为689.8 mg.kg-1,其95%可信限为607~788 mg.kg-1;AL能够显著降低两种肝损伤模型小鼠的血清转氨酶水平,减轻肝细胞病变。结论:苦豆子提取物对实验性肝损伤具有一定的保护作用。
OBJECTIVE: To determine the LD50 of intragastric administration of bitter bean extract (AL) mice and to investigate its effect on experimental hepatic injury. Methods: The median lethal dose and the 95% confidence limit were calculated with the modified fistula method. A chemical liver injury model was established by intraperitoneal injection of D-galactosamine (GaLN) and an immunological liver injury model was established by tail vein injection of mice injected with BCG plus lipopolysaccharide. , Detect serum transaminase levels in mice and take their liver for histopathological examination. RESULTS: The LD50 of oral administration of AL mice was 689.8 mg.kg-1, with a 95% confidence limit of 607-788 mg.kg-1; AL could significantly reduce the serum of two liver injury model mice. Transaminase levels reduce liver cell pathology. Conclusion: Sophora alopecuroides extract has protective effect on experimental hepatic injury.