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Protein immunoblotting demonstrated that six Chinese strains of Spotted fever group (SFG) rickettsiae isolated from northern China shared the identical antigenic polypeptides with Rickettsia sibirica (strains 246 and 232) and differed from other SFG rickettsiae. The various Species of other SFG rickettsiae exhibited serologically distinct epitopes as well as many cross reactive epitopes in protein immunoblotting. All SFG rickettsiae examined in this study had major antigenic polypeptides in the molecular size range of 113-160 kDa.