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腹膜内脓肿多由需氧菌和厌氧菌所引起,对这些单独或复合因素引起的腹膜内环境的变化迄今无满意解释。作者对此进行了研究。用350～400g雄性Sprague-Bawley鼠作实验,插入22号导管经腹壁至鼠腹腔并连接氧分压张力机。在实验初和动物杀死前用标准pH探针和SA720pH测量器测量腹膜内PH。细菌经普通培养后在37℃孵化18小时并以每分钟2400转速离心12分钟,再经稀释和培养平皿计算确定细菌数量。无菌粪便以硫酸钡悬浮液处理制成。结果用统计学方法处理。实验分:对照组(1ml生理盐水),E.Coli组(EC),脆弱拟杆菌组(BF),佐剂组(SFA),EC+BF组,EC
Intraperitoneal abscess is mostly caused by aerobic and anaerobic bacteria. So far, there are no satisfactory explanations for the changes of intraperitoneal environment caused by these single or multiple factors. The author studied this. Experiments were performed with 350-400 g male Sprague-Bawley rats. Catheter No. 22 was inserted through the abdominal wall into the abdominal cavity of rats and oxygen pressure tensioner was connected. Intraperitoneal pH was measured at the beginning of the experiment and before the animals were killed using a standard pH probe and a SA720 pH meter. Bacteria were incubated at 37 ° C for 18 hours after ordinary culture and centrifuged at 2400 rpm for 12 minutes. The number of bacteria was determined by dilution and culture. Sterile excrement is treated with barium sulfate suspension. The results were statistically analyzed. The experimental points were as follows: control group (1ml normal saline), E.coli group (EC), Bacteroides fragilis group (BF), adjuvant group (SFA), EC + BF group