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目的:观察石杉碱甲(Hup-A)对海马CA1锥体神经元兴奋性突触传递的影响,以探讨其增强学习记忆功能的神经细胞电生理机制。方法:应用大鼠海马脑片CA1锥体神经元细胞内记录技术,观察Hup-A对大鼠海马CA1锥体神经元膜电性质和刺激Schaffer侧支诱发的兴奋性突触后电位(EPSP)的影响。结果:(1)Hup-A(1μmol/L)灌流15min对CA1锥体神经元的膜电性质没有显著性影响。(2)Hup-A(0.3～3.0μmol/L)浓度依赖性使EPSP幅度升高、时程延长、曲线下面积增大,该作用可被阿托品(10μmol/L)预处理取消。(3)Hup-A对外源性谷氨酸诱导的去极化反应无明显影响。结论:Hup-A可增强CA1锥体神经元的兴奋性突触传递,其增强突触传递作用与M型乙酰胆碱受体激动有关。
OBJECTIVE: To observe the effect of huperzine A on the excitatory synaptic transmission in hippocampal CA1 pyramidal neurons in order to investigate the electrophysiological mechanism of neural cells that enhance learning and memory. Methods: The hippocampal CA1 pyramidal neurons were recorded in hippocampal slices of rat hippocampal CA1 pyramidal neurons to observe the membrane electrical properties of hippocampal CA1 pyramidal neurons and stimulate the Schaffer collaterals-induced excitatory postsynaptic potential (EPSP) Impact. Results: (1) Hup-A (1μmol / L) for 15min had no significant effect on the membrane electrical properties of CA1 pyramidal neurons. (2) The concentration dependence of Hup-A (0.3-3.0μmol / L) increased the amplitude of EPSP, prolonged the duration and increased the area under the curve. This effect could be canceled by pretreatment with atropine (10μmol / L). (3) Hup-A had no significant effect on exogenous glutamate-induced depolarization. CONCLUSION: Hup-A can enhance the excitatory synaptic transmission in CA1 pyramidal neurons, and its enhancement of synaptic transmission is related to the activation of acetylcholine receptor.