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目的某地区1月内连续地发生3起由肠炎沙门菌引起的食源性疾病,应用脉冲场凝胶电泳分型技术对疫情分离菌株进行分析,为查找传染源和传播途径,控制疫情发展提供依据。方法运用限制性内切酶XbaⅠ,对3起疫情中分离的7株肠炎沙门菌进行脉冲场凝胶电泳(PFGE)分子分型,BioNumerics V4.0软件UPGMA方法进行聚类分析。结果 3起疫情中分离的7株肠炎沙门菌交叉分为3个基因型,其中分别从疫情1、2、3中的4株菌同属基因型1,分别从疫情1、2中分离的2株菌同属基因型2,从疫情1中分离的1株菌属基因型3。结论此地区1月内暴发的3起疫情分离菌株的PFGE型别具有相同的型别,提示该地1月内暴发的3起疫情有共同的传染来源。
Objective To detect three foodborne diseases caused by Salmonella enteritidis in a certain area in January. Pulsed-field gel electrophoresis typing technique was used to analyze the isolates. To find out the sources of infection and transmission routes and to control the development of epidemic situation in accordance with. Methods Seven Salmonella enteritidis isolated from three outbreaks were subjected to PFGE molecular typing using the restriction endonuclease Xba Ⅰ. BioNumerics V4.0 software UPGMA was used for cluster analysis. Results The three isolates of Salmonella enteritidis isolated from the three outbreaks were divided into three genotypes. Among them, four isolates from the outbreaks 1, 2, and 3 belong to genotype 1, and 2 isolates from outbreaks 1 and 2, respectively Mycobacterium belongs to genotype 2, and 1 strain of genus 3 isolated from outbreak 1. Conclusion The PFGE patterns of the three isolates isolated in January in this area are of the same type, suggesting that there were common sources of infection among the three outbreaks in January.