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试验采用正交实验的方法,确定了微生态制剂的使用方式、使用间隔时间、不同菌种的比例和使用浓度的最佳方案,以提高效率、降低成本。结果表明,微生态制剂能显著提高牙鲆幼鱼消化道的蛋白酶活性。同时在水中和饲料中使用微生态制剂,可以在短时间内大幅度提高牙鲆幼鱼消化道蛋白酶活性,最佳方案是投水兼投喂、间隔1周、1芽∶10乳、浓度为1×105个/m l;养殖水体换水频率较高时,可采用这种方案。对于换水频率较低的养殖水体,最佳方案是只在水中使用、间隔1周、10芽∶1乳、浓度为1×105个/m l。微生态制剂对牙鲆幼鱼的生长有显著的促进作用,试验组的平均体重比对照组高出26.86%。
The orthogonal experiment was used to determine the way of using the probiotics, the best time to use, the ratio of different strains and the concentration of the bacteria to improve the efficiency and reduce the cost. The results showed that the probiotics significantly increased the protease activity in the digestive tract of juvenile flounder. At the same time in the water and feed the use of probiotics, in a short period of time can significantly improve the pest juvenile digestive tract proteinase activity, the best solution is watering and feeding, interval 1 week, 1 bud: 10 milk, the concentration of 1 × 105 / ml; aquaculture water exchange rate is high, this program can be used. The best solution for aquaculture water with low water change frequency is only for use in water. One week intervals, 10 buds: 1 milk, with a concentration of 1 × 10 5 / ml. The probiotics promoted the growth of juvenile Paralichthys olivaceus. The average body weight of the experimental group was 26.86% higher than that of the control group.