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采用聚合酶链反应（ＰＣＲ）结合地高辛素标记的乙肝病毒ＤＮＡ探针（ＨＢＶＤＮＡ－Ｄｉｇ）杂交技术检测了４９份乙型肝炎患者的肝组织标本和４６份血清标本中的ＨＢＶＤＮＡ。结果表明：ＰＣＲ检出乙肝病人肝组织和血清中ＨＢＶＤＮＡ阳性率比斑点杂交高（Ｐ＜０．０５）。在２１例同时检测了肝组织和血清中ＨＢＶＤＮＡ的病人中，肝组织ＨＢＶＤＮＡ阳性率为５７．１％（１２／２１）；血清阳性率为６１．３％（１３／２１）；两者总阳性率为８０．９％（１７／２１），说明用ＰＣＲ同时检测肝组织和血中ＨＢＶＤＮＡ，可明显提高ＨＢＶＤＮＡ的检出率。
HBV DNA in 49 hepatitis B patients and 46 serum samples were detected by polymerase chain reaction (PCR) combined with digoxigenin-labeled hepatitis B virus DNA probe (HBVDNA-Dig) hybridization. The results showed that the positive rate of HBVDNA in liver tissue and serum of PCR detection was higher than that of dot blot hybridization (P <0.05). Among the 21 patients with simultaneous detection of HBVDNA in liver tissue and serum, the positive rate of HBVDNA in liver tissue was 57.1% (12/21) and the positive rate of serum was 61.3% (13/21) Rate of 80.9% (17/21), indicating that simultaneous detection of HBV DNA in liver tissue and blood by PCR can significantly improve the detection rate of HBVDNA.