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背景与目的:人体正常组织器官中,特定组织中凋亡与增殖细胞维持着动态平衡,一旦凋亡与增殖的平衡被打破,将导致细胞的恶性转化和肿瘤的发生。我们通过研究结肠癌细胞和淋巴细胞演进过程中凋亡与增殖平衡机制的变化,及其与患者生存的关系,探讨结肠癌的发生、发展规律。方法:采用低分子量DNA抽提后荧光染料染色法(Sub G1法)和Ki-67/DNA双参数法,分别定量分析凋亡群体和增殖群体。应用激光共聚焦显微镜对组织细胞的增殖进行形态学观察,Kaplan-M eier法分析生存率。结果:对照组细胞、腺瘤细胞、肿瘤组Dukes蒺A期、B期、C+D期细胞中,凋亡指数/增殖指数(apoptosis index/proliferating index,AI/PI)分别为0.45±0.19、0.30±0.07、0.29±0.11、0.28±0.10和0.26±0.07。对照组的AI/PI大于其余4组(P<0.01)。在结肠癌细胞的大小、类型、分化程度、Dukes蒺分期和淋巴结转移等临床病理特征中,AI/PI总体呈下调的趋势。正常人和肿瘤患者外周血淋巴细胞AI/PI分别为0.49±0.12和0.64±0.11。肿瘤患者淋巴细胞的AI/PI大于对照组(P<0.01)。用激光共聚焦显微镜可观察到结肠对照组、腺瘤和肿瘤组织中Ki-67的表达。根据AI/PI的中位数值0.285,将结肠癌患者分为≥0.285组和<0.285组。经log-rank检验两组患者生存时间无显著性?
BACKGROUND & OBJECTIVE: In normal human tissues and organs, the apoptosis and proliferation of cells in a given tissue maintain the homeostasis. Once the balance between apoptosis and proliferation is broken, malignant transformation and tumor occurrence will occur. We studied the occurrence and development of colon cancer by studying the mechanism of apoptosis and proliferation balance during the evolution of colon cancer cells and lymphocytes and their relationship with the survival of patients. Methods: Apoptotic population and proliferating population were quantitatively analyzed by fluorescent dye staining (Sub G1 method) and Ki-67 / DNA double-parameter method after low molecular weight DNA extraction. Morphological observation of the proliferation of the tissue cells was performed by laser confocal microscopy, and the survival rate was analyzed by Kaplan-Meier method. Results: The apoptosis index / proliferating index (AI / PI) of Dukes 蒺 A, B and C + D cells in control group, adenoma cell group and tumor group were 0.45 ± 0.19, 0.30 ± 0.07, 0.29 ± 0.11, 0.28 ± 0.10, and 0.26 ± 0.07. AI / PI in the control group was greater than the other 4 groups (P <0.01). In the clinicopathological features of colon cancer cells, such as size, type, degree of differentiation, Dukes 蒺 staging and lymph node metastasis, AI / PI generally showed a downward trend. The AI / PI of peripheral blood lymphocytes in normal and tumor patients were 0.49 ± 0.12 and 0.64 ± 0.11, respectively. The AI / PI of lymphocytes in tumor patients was higher than that in control group (P <0.01). The expression of Ki-67 in colon control group, adenoma and tumor tissues was observed by laser confocal microscopy. According to the median AI / PI of 0.285, colon cancer patients were divided into ≥0.285 group and <0.285 group. By log-rank test two groups of patients had no significant survival time?