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目的 :体外研究p5 3基因提高恶性胶质瘤HSV TK/ACV系统自杀基因治疗疗效的可行性。方法 :以腺病毒为载体转导p5 3和HSV TK基因入C6鼠胶质瘤细胞 ,给以不同浓度的ACV ,MTT法监测细胞生存率 ,TUNEL法检测凋亡。评价p5 3基因提高HSV TK/ACV系统治疗胶质瘤的疗效。结果 :p5 3明显提高鼠胶质瘤细胞对HSV TK/ACV的敏感性 ,p5 3联合HSV TK转染C6的ACVID50 (1μg·mL-1)和ID10 0 (10 μg·mL-1)较单独HSV TK转染C6的ACVID50 和ID10 0 提高 10倍 ,并可使C6细胞凋亡增加。结论 :p5 3基因可明显增强胶质瘤HSV TK/ACV系统的体外抑瘤作用
OBJECTIVE: To investigate the feasibility of using p5 3 gene to improve the efficacy of suicide gene therapy in HSV TK / ACV system of glioblastoma in vitro. Methods: The adenovirus vector was used to transduce p5 3 and HSV TK into C6 glioma cells. The cell viability was monitored by ACV and MTT at different concentrations. Apoptosis was detected by TUNEL assay. To evaluate the efficacy of p5 3 gene in the treatment of gliomas by HSV TK / ACV system. Results: p5 3 significantly increased the sensitivity of glioma cells to HSV TK / ACV. Compared with ACVID50 (1 μg · mL-1) and ID10 0 (10 μg · mL-1) transfected with p5 3 and HSV TK, HSV TK transfection C6 ACVID50 and ID10 0 increased 10-fold, and C6 cells can increase apoptosis. Conclusion: The p5 3 gene can significantly enhance the anti-tumor effect of glioma HSV TK / ACV system in vitro