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[Objective] To discuss the methods for extraction and content determination of polysaccharides from HERBA DESMODII STYRACIFOLII and to study their scavenging activity to hydroxyl radical. [Method] Polysaccharides were extracted from HERBA DESMODII STYRACIFOLII with hot water method and then preliminarily purified, the content of which was measured by means of phenol-sulfuric acid method. In addition, ·OH scavenging activity of polysaccharides was tested. [Result] The content of polysaccharides in tested sample was 5.11%. The recovery rate was 99.46% with the RSD of 1.38% (n=5). HERBA DESMODII STYRACIFOLII polysaccharides could effectively scavenge ·OH generated from Fenton system. Moreover, the ·OH scavenging activity of polysaccharides was improved along with the increase of concentration. [Conclusion] HERBA DESMODII STYRACIFOLII polysaccharides have stronger scavenging activity to ·OH.
[Method] Polysaccharides were extracted from HERBA DESMODII STYRACIFOLII with hot water method and then preliminarily purified, the content of The results of The scavenging activity of polysaccharides was tested. [Result] The content of polysaccharides in tested samples was 5.11%. The recovery rate was 99.46% with the RSD of 1.38% (n = 5). HERBA DESMODII STYRACIFOLII polysaccharides could effectively scavenge OH generated from Fenton system. Furthermore, the · OH scavenging activity of polysaccharides was improved along with the increase of concentration. [Conclusion] HERBA DESMODII STYRACIFOLII polysaccharides have stronger scavenging activity to · OH.