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用新鲜鲩鱼胆汁灌胃诱发大鼠及小鼠的多器官衰竭（ＭＯＦ），探讨其与氧自由基的关系。实验结果：新鲜鲩鱼胆汁按比重法测其重量，小鼠ＬＤ＿（５０）为４３２０ｍｇ／ｋｇ体重；大鼠按３５００～４０００ｍｇ／ｋｇ体重灌胃，１１５只大鼠染毒后２４～１２０ｈ存活的５８只中出现ＭＯＦ率为７７．５９％；小鼠染毒后２４～４８ｈ，心、肝、肾、肺、脑及血浆内丙二醛（ＭＤＡ）含量明显高于对照组；染毒后２４ｈ血液内谷胱甘肽－过氧化物酶（ＧＳＨ－Ｐｘ）活性明显高于对照组，４８ｈ降至正常；红细胞超氧化物歧化酶（ＳＯＤ）活性在４８ｈ明显低于对照组；血液内还原型谷胱甘肽（ＧＳＨ）在染毒后１～６天呈３次抛物线变化趋势，染毒后１天升高，５天达最低值，６天开始回升，并逐渐恢复至染毒前水平。提示：用新鲜鲩鱼胆汁可制造ＭＯＦ模型，ＭＯＦ发生机制可能与氧自由基增多及抗氧化物质减少有关。
The fresh bream fish bile gavage induced rat and mouse multiple organ failure (MOF), to explore its relationship with oxygen free radicals. The results showed that: the fresh bream fish bile gravimetrically measured its weight, the mouse LD_ (50) was 4320mg / kg body weight; rats 3500 ~ 4000mg / kg body weight gavage, 115 rats exposed 24 ~ 120h after exposure The MOF in 58 rats was 77.59%. The contents of malondialdehyde (MDA) in heart, liver, kidney, lung, brain and plasma from 24 to 48 hours after exposure were significantly higher than those in the control group The activity of glutathione peroxidase (GSH-Px) in the blood was significantly higher than that in the control group and decreased to normal after 48h. The activity of superoxide dismutase (SOD) in erythrocytes was significantly lower than that in the control group at 48h. Glutathione (GSH) tended to show a parabolic curve three times from 1 to 6 days after exposure. The GSH level increased at 1 day and reached the lowest level at 5 days. The GSH began to rise after 6 days and gradually returned to the pre-exposure level. Tip: MOF model can be made of fresh bream fish bile, MOF mechanism may be related to increased oxygen free radicals and antioxidants.