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作者用熟前凝集染色体(PCC)技术和流式细胞术分别对肿瘤患者外周血细胞G_1-PCC断裂率和癌细胞DNA含量进行了检测.结果表明,肿瘤患者血细胞G_1-PCC断裂率明显升高(P<0.01);患者癌细胞DNA异倍体率为63.2%,其中食管癌为63.0%,宫颈癌为63.3%:S期细胞比率(SPF)明显升高(P<0.01):患者G_1-PCC断裂率随癌细胞DNA指数(DI)增高而增加(r=0.846,r>r_(0.01).异倍体肿瘤患者G_1-PCC断裂率明显高于二倍体患者(P<0.01).随病理组织学分级的增高,G_1-PCC断裂率、癌细胞异倍体检出率和DI值均逐渐升高.因此,对患者血细胞G_1-PCC断裂率和DNA含量同步检测,用于癌患者肿瘤恶性度和预后估计是有一定意义的.
The authors used the precancerous agglutination chromosome (PCC) technique and flow cytometry to detect the G 1 -PCC rupture rate and DNA content of cancer cells in tumor patients. The results showed that the blood cell G 1 -PCC rupture rate was significantly increased in tumor patients ( P<0.01); The patient’s cancer cell DNA aneuploidy rate was 63.2%, of which esophageal cancer was 63.0%, cervical cancer was 63.3%: S phase cell ratio (SPF) was significantly higher (P<0.01): patient G_1-PCC The rupture rate increased with increasing DNA index (DI) of cancer cells (r=0.846, r>r_(0.01). The rate of G_1-PCC rupture in patients with aneuploid tumors was significantly higher than that in diploid patients (P<0.01). With the increase of histological grade, G_1-PCC rupture rate, cancer cell heteroploid detection rate and DI value were gradually increased. Therefore, simultaneous detection of blood cell G_1 -PCC breakage rate and DNA content in patients was used to determine tumor malignancy in cancer patients. And the prognosis is of some significance.