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在大肠杆菌表达人淋巴毒素ｃＤＮＡ。将本室克隆的人淋巴毒素ｃＤＮＡ亚克隆到原核表达载体 ｐＢＶ2 2 2 0 ,温控法诱导重组菌 ,ＳＤＳ ＰＡＧＥ和Ｗｅｓｔｅｒｎ ｂｌｏｔｔｉｎｇ检测和鉴定结果 ,ＭＴＴ比色法测定重组ＬＴ活性。结果成功地表达了人淋巴毒素重组蛋白 ;重组蛋?
Expression of human lymphotoxin cDNA in E. coli. The cloned human lymphotoxin cDNA was subcloned into the prokaryotic expression vector pBV2220, and the recombinant plasmid was induced by temperature control. The results of SDS PAGE and Western blotting were detected and identified. The activity of recombinant LT was determined by MTT assay. The results successfully expressed human lymphotoxin recombinant protein; recombinant egg?