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目的探讨苯代谢物氢醌(hydroquinone,HQ)对小鼠骨髓造血干细胞(hematopoietic stem cell,HSC)中蛋白激酶B和Forkhead转录因子1(Forkhead box O1,FoxO1)的m RNA和蛋白表达的影响。方法提取7只健康8周龄SPF级雄性昆明小鼠的骨髓细胞,采用免疫磁珠法(magnetic activated cell sorting,MACS)分选出小鼠骨髓造血干细胞,分别暴露于含终浓度为0(对照)、1.25、2.5、5、10、20、40μmol/L HQ的培养基中培养24 h。检测细胞蛋白激酶B和FoxO1的m RNA及蛋白的表达。结果与对照组比较,各剂量HQ暴露组小鼠骨髓造血干细胞蛋白激酶B m RNA和20、40μmol/L HQ暴露组小鼠骨髓造血干细胞FoxO1 m RNA的表达水平均较高,差异均有统计学意义(P<0.05,P<0.01)。与对照组比较,2.5~40μmol/L HQ暴露组小鼠骨髓造血干细胞FoxO1蛋白和5、10μmol/L HQ暴露组小鼠骨髓造血干细胞蛋白激酶B蛋白的表达水平均较高,而20、40μmol/L HQ暴露组小鼠骨髓造血干细胞蛋白激酶B蛋白的表达水平均较低,差异均有统计学意义(P<0.05,P<0.01)。且随着HQ暴露剂量的升高,小鼠骨髓造血干细胞蛋白激酶B m RNA和蛋白的表达水平呈先上升后下降的趋势,FoxO1 m RNA和蛋白的表达水平呈波浪式下降的趋势。结论骨髓造血干细胞的调节可能部分依赖于蛋白激酶B和FoxO1。
Objective To investigate the effect of hydroquinone (HQ) on m RNA and protein expression of protein kinase B and Forkhead box transcription factor 1 (FoxO1) in the bone marrow hematopoietic stem cells (HSC). Methods Bone marrow cells from 7 Kunming mice of 8-week-old SPF were extracted and bone marrow hematopoietic stem cells were sorted out by magnetic activated cell sorting (MACS) ), 1.25,2.5,5,10,20,40 μmol / L HQ for 24 h. Detection of cellular protein kinase B and FoxO1 m RNA and protein expression. Results Compared with the control group, the expression levels of Bone Marrow Hematopoietic Stem Cell Protein Kinases B m RNA and FoxO1 m RNA in bone marrow hematopoietic stem cells of mice exposed to 20 and 40 μmol / L HQ were significantly higher than those of the control group Significance (P <0.05, P <0.01). Compared with the control group, the bone marrow hematopoietic stem cell FoxO1 protein and the bone marrow hematopoietic stem cell protein kinase B protein expression in mice exposed to 2.5 ~ 40μmol / L HQ were higher than those in the control group exposed to 5 ~ 10μmol / L HQ, The expression levels of protein kinase B protein in bone marrow hematopoietic stem cells of mice exposed to L HQ were lower than that of the mice exposed to L HQ (P <0.05, P <0.01). And with the increase of HQ exposure, the expression level of Bm RNA and protein in mouse bone marrow hematopoietic stem cells initially increased and then decreased, and the expression levels of FoxO1 m RNA and protein showed a downward trend. Conclusion The regulation of bone marrow hematopoietic stem cells may be partly dependent on protein kinase B and FoxO1.