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为探究溶藻菌Halomona Sp.DH-e无菌滤液对东海原甲藻的作用机制及其发酵产物安全性,测定了在该菌作用下东海原甲藻抗氧化系统的变化,同时对发酵产物进行了生物急性毒性试验.结果表明,当东海原甲藻细胞在受到溶藻菌DH-e的无菌滤液处理后,细胞活性氧(reactive oxygen species,ROS)含量会急剧上升,随着藻细胞抗氧化系统激活,藻细胞内丙二醛(MDA)含量增加,超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活性增强;溶藻菌发酵产物在96/h内对斑马鱼的半致死浓度LC50＞ 100 mg/L,发酵产物最大剂量5 000 mg/kg·bw下小鼠14 d的死亡率为0,半致死剂量LD50＞5 000mg/kg·bw,由实验结果看见溶藻菌Halomona Sp.DH-e发酵产物属低毒或无毒物质.“,”We studied the change of the antioxidant system of Prorocentrum donghaiense during lysing process treated with Halomona Sp.DH-e cell-free filtrate and the acute toxicity test of fermentation products to explore the inhibitory mechanism of DH-e on the P.donghaiense and acute toxicity of DH-e fermentation products.The results showed that the reactive oxygen species (ROS) metabolism of algae cell had an increasing trend after treated with DH-e aseptic filtrate.Then the self-protection mechanism of algal cells was started.Furthermore,of the activities of antioxidant enzymes,such as superoxide dismutase (SOD) and catalase (CAT),and Malodialdehyde (MDA) content both were increased.We found that the semi-lethal concentration was LC50 ＞ 1 00 mg/L when the zebrafish treated with fermentation products for 96h.The mortality rate of zebrafish effected by fermentation maximum dose (5 000 mg/kg · bw) was 0％ in 14 d (LD50 ＞5 000 mg/kg · bw).These results revealed that the fermentation is low-toxic.