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[目的]研究多菌灵的降解机理。[方法]通过培养试验和紫外光谱法,探讨高效降解菌株对多菌灵的降解性能及降解途径。[结果]所选用的高效菌株Raoultella sp.MBC能够以多菌灵及其降解产物为唯一碳源,在试验条件下培养72 h后,多菌灵的降解率达到100%,紫外光谱表明多菌灵经菌株降解后,分子中咪唑结构中的C=N被破坏。[结论]根据降解产物确定多菌灵的降解途径为多菌灵→2-氨基苯并咪唑→2-羟基苯并咪唑→邻苯二胺,但邻苯二胺并不是最终降解产物。
[Objective] The research aimed to study the degradation mechanism of carbendazim. [Method] The degradation and degradation of carbendazim by degrading bacteria were studied by culturing test and ultraviolet spectrometry. [Result] The selected high efficient strain Raoultella sp. MBC was able to use carbendazim and its degradation products as the sole carbon source. The degradation rate of carbendazim reached 100% after cultured for 72 h under the experimental conditions. The UV spectrum indicated that the bacteria After the degradation of the schizophyllum, the C = N in the imidazole structure of the molecule is destroyed. [Conclusion] According to the degradation products, the degradation pathway of carbendazim was carbendazim → 2-aminobenzimidazole → 2-hydroxybenzimidazole → o-phenylenediamine, but o-phenylenediamine was not the final degradation product.