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无论是无菌播种还是组培繁殖兰花,最后均有试管苗出瓶盆栽这一程序。许多人前期工作进行得很好,但往往在试管苗出瓶盆栽时碰到许多困难,导致成活率不高。这里根据个人的体会,以大花蕙兰(Cymbidiums)为例,将一些具体操作方法尽可能详细地介绍给读者,希望对促进我国兰花产业发展有些许用处。一、培养健壮的试管苗通过无菌播种(称为播种苗)和组培得到的试管苗(又称为分生苗)往往大小不齐,还有一些在培养中产生明显变异的植株或类原球茎(PLB),这就需要进行筛选和淘汰。首先挑选那些生长健壮、大小相近的苗转接到同一培养瓶中,严格淘汰产生变异的植株和 PLB。只有这样才能保证试管苗的纯正(保持母本植株的优良特性)一致。另一要点足培养条件,包括足够大小的培养瓶、充足和适宜的培养基以及适宜的光照与温度。目前,许多培养者
Whether it is aseptic sowing or tissue culture propagation orchids, and finally have a test tube plant out of the bottle pot this procedure. Many people work very well in their previous work, but often encounter many difficulties in the test tube seedling out of pots, resulting in low survival rate. Here based on personal experience, to Cymbidium (Cymbidiums), for example, some of the specific methods of operation as much as possible to introduce readers, hoping to promote the development of China’s orchid industry with some useless. First, to develop robust test-tube seedlings Plantlets (or seedlings) obtained through aseptic sowing (called sowing seedlings) and tissue culture tend to be of different sizes, with some plants or classes that have significant variability in their culture Protocorm (PLB), which requires screening and elimination. First pick those robust growth, the size of similar seedlings transferred to the same flask, strict elimination of mutated plants and PLB. The only way to ensure the purity of the plantlets (keeping the excellent characteristics of the maternal plants) is consistent. Another important point to point culture conditions, including the size of the flask, adequate and suitable medium and the appropriate light and temperature. At present, many cultivators