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该研究旨在探讨莪术油(zedoray turmeric oil,ZTO)对人肺腺癌A549细胞增殖的影响及诱导细胞凋亡作用。不同浓度莪术油作用A549细胞48 h后,采用MTT法检测细胞存活率;光学显微镜、荧光显微镜和透射电镜观察细胞形态结构;流式细胞术检测细胞周期、细胞凋亡率和线粒体膜电位(ΔΨm);Real Time RT-PCR和Western blot检测Bcl-2/Bax表达水平。结果显示,莪术油对A549细胞生长具有剂量依赖性抑制作用,莪术油作用A549细胞48 h的最佳浓度是80μg/mL,增殖抑制率为(77.462%±0.681%),显微镜下观察细胞呈明显凋亡现象,细胞凋亡率为(27.31%±0.43%),ΔΨm显著下降(P<0.01),细胞阻滞于S期和G2期;Bcl-2的表达下调,Bax的表达明显增加,Bcl-2/Bax比值显著降低(P<0.01)。提示莪术油能抑制肺腺癌A549细胞增殖,通过上调Bax下调Bcl-2诱导其凋亡。
The aim of this study was to investigate the effects of zedoray turmeric oil (ZTO) on the proliferation of human lung adenocarcinoma A549 cells and the induction of apoptosis. A549 cells were treated with different concentrations of Curcuma oil for 48 h, the cell viability was detected by MTT assay; the morphology of cells was observed by optical microscope, fluorescence microscope and transmission electron microscope; the cell cycle, apoptosis rate and mitochondrial membrane potential (ΔΨm ); Real time RT-PCR and Western blot detection of Bcl-2 / Bax expression levels. The results showed that zedoary turmeric oil had a dose-dependent inhibitory effect on the growth of A549 cells. The optimal concentration of Curcuma oil treated A549 cells for 48 h was 80 μg / mL and the proliferation inhibition rate was (77.462% ± 0.681%). The cells under microscope showed obvious (P <0.01). The cells were arrested in S phase and G2 phase. The expression of Bcl-2 was down-regulated while the expression of Bax was significantly increased -2 / Bax ratio was significantly lower (P <0.01). These results suggest that Curcuma oil can inhibit the proliferation of lung adenocarcinoma A549 cells and induce apoptosis by up-regulating Bax and down-regulating Bcl-2.