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通过投喂经甘露寡糖强化的卤虫无节幼体,探索其对半滑舌鳎稚鱼(初始体长为1.4 cm左右)生长性能、肠道发育和非特异性免疫水平的影响。试验设两个处理组(对照组和甘露寡糖组),分别投喂经裂壶藻或甘露寡糖+裂壶藻强化的卤虫无节幼体。试验进行34 d,每17 d取样1次。结果显示,17 d时甘露寡糖组体长显著大于对照组(P<0.05)。两组间的特定生长率和存活率无显著性差异(P>0.05)。RNA/DNA和protein/DNA的比值在17 d时两组间均无显著性差异(P>0.05);34 d时甘露寡糖组的RNA/DNA和protein/DNA比值均大于对照组。甘露寡糖组17 d时淀粉酶比活力和34 d时胰蛋白酶比活力显著高于对照组(P<0.05)。甘露寡糖组的过氧化物歧化酶、过氧化氢酶、过氧化物酶和溶菌酶活性均显著高于对照组(P<0.05)。甘露寡糖组的微绒毛长度和皱襞高度显著大于对照组(P<0.05);黏膜厚度大于对照组,但无显著性差异(P>0.05)。试验结果表明,甘露寡糖可以提高半滑舌鳎稚鱼肠道发育和非特异性免疫水平。
The effects of growth, gut development and nonspecific immunity on the growth of juvenile halibut, juvenile (initial body length of 1.4 cm), were explored by feeding Artemia nauplii enriched with mannose oligosaccharides. Two treatment groups (control group and mannooligosaccharides group) were set up and fed with Artemia nauplii enhanced by Pythium microphylla or Mannooligosaccharides + Pyrenophora. The trial ran for 34 days and sampled every 17 days. The results showed that the body length of manna oligosaccharide group was significantly larger than that of the control group at 17 days (P <0.05). There was no significant difference in specific growth rate and survival between the two groups (P> 0.05). The ratio of RNA / DNA to protein / DNA was not significantly different between the two groups at 17 days (P> 0.05). On the 34th day, the ratio of RNA / DNA and protein / DNA in mannan oligosaccharides group was higher than that in control group. The specific activity of amylase and the specific activity of trypsin on the 34th day in the mannan oligosaccharide group were significantly higher than those in the control group on the 17th day (P <0.05). The activities of superoxide dismutase, catalase, peroxidase and lysozyme in the mannan oligosaccharide group were significantly higher than those in the control group (P <0.05). The length of microvilli and folds in the mannose oligosaccharides group were significantly higher than those in the control group (P <0.05). The mucosal thickness was greater than that in the control group, but no significant difference (P> 0.05). The results showed that mannan oligosaccharides can improve the intestinal development and non-specific immunity of juvenile smooth tongue juveniles.