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目的采用形态学、免疫学和细胞遗传学（ＭＩＣ）分型体系对５２例急性白血病进行综合分型。方法按照ＦＡＢ标准进行形态学分型。运用间接免疫荧光法标记活细胞膜表面分化抗原（ＣＤ），确定细胞属性。采用２４小时培养法或直接法制备染色体标本。结果形态学分型、免疫学分型与ＭＩＣ分型符合率分别为８６．５％和９８．１％。成功制备的３５例染色体标本中异常核型２４例，其中特异性异常１７例。Ｌｙ＋－ＡＭＬ预后较好，而Ｍｙ＋－ＡＬＬ预后较差；Ｍ２、Ｍ３、Ｍ４ｅｏ伴特异性核型ＣＲ率较高，预后较好；而３例伴ｔ（９；２２）的Ｌ２均未获ＣＲ。结论ＭＩＣ分型可提高ＡＬ的分型准确性，具有指导临床、提示预后的意义
Objective To classify 52 cases of acute leukemia using morphological, immunological and cytogenetic (MIC) typing systems. Methods The morphological classification was performed according to FAB criteria. Indirect immunofluorescence was used to label living cell membrane surface differentiation antigen (CD) to determine cell properties. Chromosomal specimens were prepared using the 24-hour culture method or the direct method. Results The coincidence rates of morphological typing, immunological typing and MIC typing were 86.5% and 98.1%, respectively. Of the 35 chromosomal specimens successfully prepared, 24 were abnormal karyotypes, of which 17 were specific abnormalities. The prognosis of Ly+-AML is better, but the prognosis of My+-ALL is poorer; the CR rate of M2, M3, M4eo with specific karyotype is higher, and the prognosis is better; and 3 cases of L2 with t(9; 22) are not given CR. . Conclusion MIC classification can improve the classification accuracy of AL, and has the significance of guiding clinical and prompting prognosis.