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目的比较3种不同化学修饰的水分散性碳纳米管[磷酰胆碱(phosphoryl choline,PC)修饰碳纳米管(CNT-PC)、聚乙二醇修饰碳纳米管(CNT-PEG)、磷酰胆碱和聚乙二醇修饰碳纳米管(CNT-PEG-PC)]在大鼠体内的急性毒性、分布和其他生物效应。方法将144只清洁级SD大鼠按体重随机分为8组,分别为对照(PBS缓冲溶液)组和12.5、25、50、70、100、140、200 mg/kg CNTs染毒组,每组6只,雌雄各半。采用尾静脉注射方法进行1次性染毒,注射容量为10 ml/kg。观察动物反应及死亡情况。染毒7 d后,检测全血淋巴细胞数量和血清白蛋白(ALB)、丙氨酸氨基转移酶(ALT)、肌酐(Cr)含量,并进行组织病理学观查。结果当3种碳纳米管修饰物的注射剂量>25 mg/kg时,与有PC基团修饰的CNTs相比,CNT-PEG更容易导致大鼠的窒息死亡;含有PEG基团结构的CNTs在大鼠血液循环系统中滞留时间更长,而CNT-PC则更容易进入肺、肝和脾组织中,并且分散良好;各剂量CNT-PC、CNT-PEG和CNT-PEG-PC染毒组大鼠血清ALB、ALT和Cr含量间比较,差异无统计学意义。CNT-PC染毒大鼠血液淋巴细胞数量随注射剂量的升高而逐渐增加;且与对照组比较,50～200 mg/kg的CNT-PC染毒组大鼠血液淋巴细胞数量较多,差异均有统计学意义(P<0.05)。经3种修饰过的CNTs染毒后,在大鼠体内均未发现炎症和病变。结论 3种CNTs修饰物都没有表现出明显的急性毒性;但由于修饰基团不同化学结构的作用,其在大鼠体内表现出不同的分布和生物效应。
OBJECTIVE To compare the effects of three different chemically modified water-dispersible carbon nanotubes (CNT-PC, CNT-PEG, P Acute toxicity, distribution and other biological effects of acetylcholine and polyethylene glycol modified carbon nanotubes (CNT-PEG-PC) in rats. Methods One hundred and fourteen clean SD rats were randomly divided into 8 groups according to body weight: control (PBS buffer) group and 12.5, 25, 50, 70, 100, 140 and 200 mg / kg CNTs exposure groups, 6, half male and half female. A single intravenous injection of tail vein injection method, the injection capacity of 10 ml / kg. Observe animal reactions and deaths. After 7 days of exposure, the amount of whole blood lymphocytes and serum albumin (ALB), alanine aminotransferase (ALT) and creatinine (Cr) were measured and histopathological examination was performed. Results CNT-PEG was more likely to cause asphyxiation in rats than PC-modified CNTs at the injection dose of> 25 mg / kg. The CNTs containing PEG-containing The retention time of the blood circulatory system in rats was longer, while CNT-PC was more likely to enter the lung, liver and spleen tissues, and dispersed well; large doses of CNT-PC, CNT-PEG and CNT-PEG-PC Serum ALB, ALT and Cr content between the comparison, the difference was not statistically significant. The number of blood lymphocytes in CNT-PC-treated rats increased gradually with the increase of injection dose. Compared with the control group, the numbers of blood lymphocytes in CNT-PC-treated rats at 50 ~ 200 mg / kg were significantly increased All were statistically significant (P <0.05). After three kinds of modified CNTs were infected, no inflammation and lesions were found in rats. CONCLUSION: All three CNTs modifiers show no obvious acute toxicity. However, due to the different chemical structure of the modified groups, they show different distribution and biological effects in rats.