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分别从光密度(OD值)、p H值、Ca2+浓度和无机碳含量(DIC)4个方面对环境中生物对微生物水泥矿化过程的影响机理展开研究。结果表明:虽然环境中的微生物能为Ca CO3晶体的沉积提供额外的成核位置,但其自身的生长繁殖、呼吸作用会消耗大量的营养物质,导致试样中p H值和CO32–浓度的降低,不利于碳酸钙沉积的析出。造成在工程应用中需要生成大体量的Ca CO3时,速率、效率明显低于无环境中生物时的情形。因此,在微生物水泥技术实际应用中,必须考虑环境中已有生物的影响。试样中仅加入产脲酶菌就可以达到持续生成Ca CO3的目的,建议不加入营养物质以降低刺激环境中生物非矿化反应的风险。
The influence mechanism of the organism on microbial mineralization in the environment was studied from four aspects of OD value, p H value, Ca 2+ concentration and inorganic carbon content (DIC). The results show that although the microorganisms in the environment can provide additional nucleation sites for the deposition of CaCO 3 crystals, their own growth and reproduction respiration consume a large amount of nutrients, resulting in p H values and CO 32 -concentrations in the sample Reduce, is not conducive to precipitation of calcium carbonate deposition. Resulting in the need to generate a large amount of CaCO3 in engineering applications, the rate, the efficiency is significantly lower than in the absence of biological organisms. Therefore, the practical application of microbial cement technology, we must consider the impact of existing organisms in the environment. Only adding urease into the sample can achieve the purpose of continually generating CaCO3. It is suggested that no nutrients be added to reduce the risk of stimulating non-mineralized biological reactions in the environment.