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准确测定血清中IL 2活性对评价用重组IL-2进行全身治疗的作用和毒性很重要。以前观察者们采用两种方法确定人重组IL-2治疗性注射后的血清IL-2水平。一种方法是将血清标本在56℃孵育30分钟(加热灭活)以便消除天然存在于人血清中的IL-2抑制因子活性;另一种方法是将血清进行系列稀释使其天然IL-2抑制因子活性在用生物方法测定IL-2活性时不起作用。作者在文中比较了这两种方法以便更准确地测定血清中IL-2水平。作者自病人或健康供血者获得血清标本,健康人血清在体外加入重组IL-2,在生物活性测定之前取相同的标本分别在56℃
Accurate determination of IL2 activity in serum is important for evaluating the efficacy and toxicity of systemic treatment with recombinant IL-2. Two methods were used by previous observers to determine serum IL-2 levels following human recombinant IL-2 injections. One approach is to incubate serum samples at 56 ° C for 30 minutes (heat inactivated) to eliminate IL-2 inhibitory factor activity naturally present in human serum; another approach is to serially dilute sera to make native IL-2 Inhibitor activity does not play a role in the biological assay of IL-2 activity. The authors compare these two methods in order to more accurately determine serum IL-2 levels. The authors obtained serum samples from patients or healthy donors, and healthy human serum added recombinant IL-2 in vitro. The same samples were taken at 56 ° C. before bioactivity determination