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以细胞分泌功能和存活率为指标，观察了醋酸棉酚对无血清培养的大鼠黄体和人蜕膜、滋养层细胞的直接损伤作用和可能的作用途径。结果表明：醋酸棉酚对黄体细胞有直接杀伤作用，ＬＤ５０为：１６（０４～２９）μｇ·ｍｌ－１。在非致死剂量（０５μｇ·ｍｌ－１）下，醋酸棉酚显著抑制黄体细胞基础孕酮分泌，但不能显著抑制ｈＣＧ，Ｆｏｒｓｋｏｌｉｎ的促孕酮作用和３βＨＳＤ的活性。此外，醋酸棉酚对蜕膜细胞和滋养层细胞也有直接杀伤作用，其ＬＤ５０分别为：３５（０４～６６）μｇ·ｍｌ－１，４１（０６～７６）μｇ·ｍｌ－１。本文结果提示，直接杀伤黄体，蜕膜和滋养层细胞并影响孕酮合成是醋酸棉酚抗生育的重要作用环节
Taking the secretory function and survival rate of cells as indexes, we observed the direct damage effect of gossypol acetate on serum-free rat luteal cells, human decidua and trophoblast cells and possible pathways. The results showed that gossypol acetate had a direct killing effect on the corpus luteum cells with LD50 of 16 (04 ~ 29) μg · ml-1. At non-lethal dose (05μg · ml-1), gossypol acetate significantly inhibited the secretion of basal progesterone in luteal cells, but could not significantly inhibit the progestogen effect of hCG and Forskolin and the activity of 3β-HSD. In addition, gossypol acetate also has a direct killing effect on decidual cells and trophoblast cells with LD50 of 3.5 (0.6-6.0) μg · ml -1,41 (06-7 6) μg · ml-1. The results suggest that direct killing of the corpus luteum, decidua and trophoblast cells and affect the synthesis of progesterone acetate is an important part of anti-fertility aspects of gossypol