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以双单克隆抗体夹心ＥＬＩＳＡ法检测８１例慢性乙肝患者（慢性迁延性肝炎１０例，慢性活动性肝炎３８例，肝炎后肝硬化１３例，慢性重症肝炎２０例）血浆与外周血单个核细胞培养上清液中的ｓＩＬ－２Ｒ。结果显示，不同临床类型的慢性乙肝血浆ｓＩＬ－２Ｒ水平均明显高子正常对照组（Ｐ均＜０．０１）。除慢活肝与肝炎后肝硬化的ｓＩＬ－２Ｒ水平未见明显差异外（Ｐ＞０．０５），其余各组差异显著（Ｐ＜０．０１）。而不同临床类型的慢性乙肝之间及与正常对照组间外周血单个核细胞培养上清液中的ｓＩＬ－２Ｒ水平差异无显著性（Ｐ均＞０．０５）。提示不同临床类型的慢性乙肝都存在免疫效应细胞的过度活化，慢性乙肝患者的ＰＢＭＣ体外释放ｓＩＬ－２Ｒ的能力无异常。
Monoclonal antibody sandwich ELISA was used to detect the plasma and peripheral blood mononuclear cells in 81 chronic hepatitis B patients (10 chronic persistent hepatitis, 38 chronic active hepatitis, 13 posthepatitic cirrhosis and 20 chronic severe hepatitis) Supernatant of sIL-2R. The results showed that different clinical types of chronic hepatitis B plasma sIL-2R levels were significantly higher in the normal control group (P all <0.01). The levels of sIL-2R in chronic liver cirrhosis and hepatitis cirrhosis were not significantly different (P> 0.05), but the other groups had significant difference (P <0.01). However, the levels of sIL-2R in peripheral blood mononuclear cell culture supernatants were not significantly different between different clinical types of chronic hepatitis B and normal controls (all P> 0.05). Prompted that different clinical types of chronic hepatitis B immune cells exist overactivation of chronic hepatitis B PBMC in vitro release of sIL-2R no abnormalities.