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目的：降低肿瘤疫苗细胞的致瘤性。方法：观察正常小鼠活化 Ｂ 淋巴细胞和小鼠 Ａ Ｌ Ａ９７０２ 肺癌细胞的杂交瘤苗细胞（ Ｆ Ｌ Ｂ２ Ｃ 、 Ｆ Ｌ Ｂ６ Ｇ） 的体外生长曲线，软琼脂集落形成及体内成瘤情况。结果： Ｆ Ｌ Ｂ２ Ｃ 、 Ｆ Ｌ Ｂ６ Ｇ 较 Ａ Ｌ Ａ９７０２ 细胞生长明显缓慢，细胞倍增时间延长， Ｆ Ｌ Ｂ２ Ｃ 不能在软琼脂中形成集落， Ｆ Ｌ Ｂ６ Ｇ 在软琼脂中形成集落明显减少， Ｆ Ｌ Ｂ２ Ｃ 、 Ｆ Ｌ Ｂ６ Ｇ 均不能在小鼠体内成瘤。结论：通过与正常细胞融合的小鼠肺癌疫苗细胞的致瘤性明显减弱甚至消失，说明这样的疫苗细胞具有潜在的临床应用价值。
Objective: To reduce the tumorigenicity of tumor vaccine cells. METHODS: The in vitro growth curves of hybridoma cells (F L B2 C, F L B6 G) of normal mouse activated B lymphocytes and mouse A L A9702 lung cancer cells were observed, as well as soft agar colony formation and tumor formation in vivo. Results: The growth of F L B2 C and F L B6 G cells was slower than that of A L A9702 cells. The cell doubling time was prolonged. F L B2 C could not form colonies in soft agar, and F L B6 G significantly decreased colony formation in soft agar. F L B2 C and F L B6 G were not tumorigenic in mice. Conclusion: The tumorigenicity of mouse lung cancer vaccine cells fused with normal cells was significantly weakened or even disappeared, indicating that such vaccine cells have potential clinical application value.