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目的:了解2010年-2011年间上海市浦东新区由副溶血性弧菌引起的腹泻病的流行特征及副溶血性弧菌的病原特征。方法:采集监测点医院腹泻病例的肛拭标本,登记病例信息。依据《感染性腹泻诊断标准》WS271-2007进行分离培养、生化鉴定、血清分型;用WHO推荐的K-B纸片法进行药敏试验。结果:2年间共检测腹泻病例样品5590件,检出副溶血性弧菌299株,检出率为4.99%。检出高峰7月-9月份;年龄分布4岁～81岁,男女比例接近1∶1;以临海的东部地区居多。血清分型方面,共分出8个血清群,30个血清型,其中O3:K6型检出186件,占62.21%,为主要血清型;药敏试验发现氨苄西林耐药率达到99.33%以上;对阿莫西林/克拉维酸等4种抗生素则完全敏感。结论:基本摸清了浦东新区腹泻病例副溶血弧菌的流行特点、血清型、耐药性,为疾病的诊治和预防提供科学依据。
Objective: To understand the epidemiological characteristics of diarrheal diseases caused by Vibrio parahaemolyticus and pathogenic characteristics of Vibrio parahaemolyticus in Pudong New Area, Shanghai from 2010 to 2011. Methods: The samples of anal swab in hospital diarrhea cases were collected and the case information was registered. According to “diagnostic criteria for infectious diarrhea” WS271-2007 for isolation and culture, biochemical identification, serotyping; drug recommended by the WHO K-B method for susceptibility testing. Results: A total of 5590 cases of diarrhea were detected in 2 years and 299 strains of Vibrio parahaemolyticus were detected, the detection rate was 4.99%. The peak was detected in July-September; the age distribution ranged from 4 to 81 years old, and the ratio of men and women was close to 1: 1. Mostly in the eastern part of Linhai. In terms of serotyping, 8 serogroups and 30 serotypes were isolated, of which 186 were detected by O3: K6 type, accounting for 62.21% of the total serotypes. The susceptibility test showed that the resistance rate of ampicillin reached over 99.33% ; Amoxicillin / clavulanic acid and other four kinds of antibiotics are completely sensitive. Conclusion: The epidemic characteristics, serotypes and drug resistance of Vibrio parahaemolyticus in the diarrhea cases of Pudong New Area were basically ascertained, providing a scientific basis for the diagnosis, treatment and prevention of the disease.