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目的:对本地区食物中毒的沙门菌的分子分型及耐药性进行分析。方法:菌株分离、生化鉴定和血清型确定参照GB/T4789.4-2003方法进行,采用PFGE进行分子分型,利用药敏卡通过仪器对菌株进行药敏试验。结果:从12份标本中,检出8株塔克松尼沙门菌,经PFGE分型,8株源于食物中毒病人和食物标本的塔克松尼沙门菌其带型一致,为相同菌株。对8株塔克松尼沙门菌进行了21种抗生素的药敏试验,受试菌对大多数常用的头孢类和喹酮类抗生素敏感。结论:PFGE是一种非常有效的分子分型方法,建立细菌PFGE指纹图谱数据库及PulseNet,对于控制食源性疾病非常重要。
Objective: To analyze the molecular typing and drug resistance of Salmonella in food poisoning in this area. Methods: Isolation of strains, biochemical identification and determination of serotypes were carried out according to the method of GB / T4789.4-2003. PFGE was used for molecular typing. Drug susceptibility test was performed on the strains by using drug susceptibility card. Results: Among the 12 specimens, 8 strains of Toxoplasma gondii were detected. According to PFGE typing, 8 strains of Toxoplasma gonorrhoeae isolated from food poisoning patients and food samples were identical in their banding patterns and were the same strains. Antibiotic susceptibility tests of 21 antibiotics were carried out on eight strains of Tox., And the test strains were sensitive to most commonly used cephalosporins and quinolones. Conclusion: PFGE is a very effective molecular typing method. The establishment of PFGE fingerprinting database and PulseNet is very important for the control of foodborne diseases.