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目的探讨尿液中透明质酸酶(HAase)和核基质蛋白22(NMP-22)联合检测在膀胱肿瘤诊断中的价值。方法采用ELISA方法分别检测40例健康人(对照组)、60例膀胱肿瘤(肿瘤组)患者手术前后尿HAase、NMP-22含量,并计算两指标术前单独检测及联合检测的灵敏度、特异性和准确度。结果肿瘤组手术前后尿HAase含量分别为(77.5±21.8)、(51.5±14.7)mg/ml,对照组为(23.3±6.3)mg/ml,术前含量高于术后(P<0.05)以及对照组(P<0.01)。肿瘤组手术前后NMP-22含量分别为(26.4±6.6)、(17.6±4.4)ng/ml,对照组为(8.1±2.1)ng/ml,术前含量高于术后(P<0.05)以及对照组(P<0.01)。术前尿HAase检测的灵敏度、特导性及准确度分别为89.1%、86.4%及86.1%,术前尿NPM-22检测的灵敏度、特异性及准确度分别为74.3%、85.2%及78.6%,而HAase与NMP-22联合检测的灵敏度、特异性及准确度分别为95.1%、80.9%及90.3%。结论尿HAase与NMP-22联合检测诊断膀胱肿瘤的灵敏度、特异性和准确度均较高,是临床诊断与预后判断的较好指标。
Objective To investigate the diagnostic value of combined detection of hyaluronidase (HAase) and nuclear matrix protein 22 (NMP-22) in urinary bladder tumors. Methods The contents of urinary HAase and NMP-22 in 40 healthy people (control group) and 60 patients with bladder cancer (tumor group) before and after operation were measured by ELISA. The sensitivity and specificity And accuracy. Results The urinary HAase contents in the tumor group before and after operation were (77.5 ± 21.8) and (51.5 ± 14.7) mg / ml, respectively, and those in the control group were (23.3 ± 6.3) mg / Control group (P <0.01). The levels of NMP-22 in the tumor group before and after operation were (26.4 ± 6.6) and (17.6 ± 4.4) ng / ml, respectively, and those in the control group were (8.1 ± 2.1) ng / Control group (P <0.01). The sensitivity, specificity and accuracy of preoperative urinary HAase detection were 89.1%, 86.4% and 86.1% respectively. The sensitivity, specificity and accuracy of preoperative urinary NPM-22 detection were 74.3%, 85.2% and 78.6%, respectively , While the sensitivity, specificity and accuracy of HAase and NMP-22 detection were 95.1%, 80.9% and 90.3% respectively. Conclusion The sensitivity, specificity and accuracy of urinary HAase and NMP-22 in the diagnosis of bladder cancer are both high, which is a good indicator of clinical diagnosis and prognosis.