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本文介绍了利用低浓度的甲醛及二硫苏糖醇,从正常的及BCG激活的豚鼠、小鼠腹腔渗出巨噬细胞制备具有巨噬细胞表面质膜受体活性的微小泡的方法;并对分离出来的巨噬细胞微小泡的形态特点、生物活性及发泡后的巨噬细胞活性进行了检测。将收获的巨噬细胞置于含有1mM
This article describes a method for preparing microvesicles with macrophage cell surface plasmalemma receptor activity by exudating macrophages from normal and BCG-activated guinea pigs and mice using low concentrations of formaldehyde and dithiothreitol. Morphological characteristics, biological activity and macrophage activity of isolated macrophages were examined. The harvested macrophages were placed in a buffer containing 1 mM