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白喉类毒素在pH9.4的0.1MNH_4Cl-NH_3·H_2O底液中改性,产生还原峰和氧化峰,峰电位分别为-1.52V和-1.46V(vs.SCE)。用单扫示波极谱法得到还原(或氧化)峰的峰电流与白喉类毒素浓度在2.5—180μg/ml范围内均呈良好线性关系。用微分脉冲极谱法可检测浓度低至0.3μg/ml的白喉类毒素。由特异性免疫结合反应,微分脉冲极谱法测得白喉类毒素的峰电流降低值与白喉抗毒素浓度在2.5×10~(-6)—5×10~(-5)IU/ml范围内呈正比关系,可测定白喉抗毒素浓度。方法简便、快速、灵敏度较高,可均相测定。用氧化峰电流测定百日咳菌苗-白喉-破伤风类毒素混合剂中白喉类毒素的含量,结果满意。
Diphtheria toxoid was modified at pH 9.4 in 0.1 M NH 4 Cl-NH 3 · H 2 O at the end of solution to give the reduction and oxidation peaks at -1.52 V and -1.46 V (vs.SCE), respectively. The peak current of reduction (or oxidation) peak obtained by single sweep oscillopolarography showed a good linear relationship with diphtheria toxoid concentration in the range of 2.5-180 μg / ml. Diphtheria toxoid at concentrations as low as 0.3 μg / ml can be detected by differential pulse polarography. The peak current reduction of diphtheria toxoid was positively correlated with the diphtheria antitoxin concentration in the range of 2.5 × 10 -6 -6 -5 -5 10 -5 IU / ml by specific immune binding reaction and differential pulse polarography. Ratio, diphtheria anti-toxin concentration can be determined. The method is simple, rapid, sensitive and can be measured in homogeneous phase. Diphtheria toxoid content in pertussis vaccine-diphtheria-tetanus toxoid mixture was determined by oxidation peak current with satisfactory results.