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本文报道了在大豆自花授粉后,利用其形成的花粉管通道,将外源DNA直接导入栽培大豆的研究结果。 通过对10组大豆实验材料进行外源DNA导入的结果看出:转化的后代主要表现在熟期、株型、花色、种皮包、百粒重和蛋白质含量的变异;变异的D_2代单株或株系的过氧化物同功酶酶谱和酶活性显示了明显的差异,其中发生“疯狂分离”的单株,其酶带显示不规律;表型变异不明显而蛋白质含量高于受体的8701组合的D_2代各株系,其酶活性明显增强,并主要表现在A_1和B区。 实验结果表明:外源DNA片段直接导入受体植物卵细胞、合子或早期胚细胞,部分片段可以被受体细胞DNA整合和表达。还表明:利用花粉管通道途径来实现外源DNA直接导入大豆,进行大豆种质创新和品质改良也是可能的。
This paper reports the results of the direct introduction of exogenous DNA into cultivated soybean using pollen tube channels formed after self-pollination of soybean. The result of introducing foreign DNA into 10 groups of soybean materials showed that the progenies of the transformation were mainly in the variation of maturity, plant type, flower color, seed coat weight, 100-grain weight and protein content. The isoenzyme zymogram and enzyme activity of the strains showed obvious differences. Among them, the strains with “crazy separation” showed irregular bands and the phenotypic variation was not obvious but the protein content was higher than that of the receptor 8701 combination of D_2 generation of each strain, its enzyme activity was significantly enhanced, and mainly in the A_1 and B area. The experimental results show that the exogenous DNA fragments can be directly introduced into the recipient plant egg cells, zygotes or early embryonic cells, and some of the fragments can be integrated and expressed by the recipient cell DNA. It also shows that it is also possible to use the pollen tube pathway to direct the introduction of exogenous DNA into soybean for soybean germplasm innovation and quality improvement.