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以大花飞燕草幼苗为试材,研究了干旱胁迫及复水条件对大花飞燕草幼苗膜脂过氧化和保护酶活性、渗透物质等生理特性的影响。结果表明:随干旱胁迫时间的延长,细胞膜透性和丙二醛(MDA)含量不断增加,在干旱胁迫的第20天,细胞膜透性和MDA含量均达到最大,此时植物受害严重。叶片中脯氨酸(Pro)含量迅速增加,复水后又开始下降,膜透性得到了一定的恢复。可溶性蛋白质含量不断下降。复水后,植物的可溶性蛋白质含量有所升高。当胁迫20d时,其体内可溶性蛋白质含量最低。随着时间的推移,干旱胁迫下植株体内的过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性均呈先上升后下降的趋势,复水后,POD、SOD活性呈缓慢升高的趋势。第10天时,POD、SOD活性达到最大值,说明此时植物自身有较高的清除活性氧的能力。
The experiment was conducted to study the effects of drought stress and rehydration conditions on the lipid peroxidation and the activities of protective enzymes, osmolytes and other physiological properties of Pedicularis grandis larvae seedlings. The results showed that the cell membrane permeability and the content of malondialdehyde (MDA) increased with the prolongation of drought stress. The membrane permeability and MDA content reached the maximum at the 20th day of drought stress, and the plants were severely damaged. Proline (Pro) content in leaves increased rapidly, rehydration and then began to decline, the membrane permeability has been a certain recovery. Soluble protein content continues to decline. After rehydration, the soluble protein content of plants increased. When the stress 20d, the body’s lowest soluble protein content. With the passage of time, the activities of peroxidase (POD) and superoxide dismutase (SOD) in plants increased first and then decreased under drought stress. After rehydration, the activities of POD and SOD increased slowly High trend. On the 10th day, the activity of POD and SOD reached the maximum value, indicating that the plant itself had a higher ability to scavenge reactive oxygen species.