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目的 探讨建立兔肝VX2 肿瘤模型 ,为该模型的应用提供实验依据。方法 采用VX2 细胞株复制兔肝肿瘤模型 ,并改进其制作方法 ,观察其肿瘤的生长过程 ,并检测肝功能谷丙转氨酶 (ALT)、谷草转氨酶 (AST)及总胆红素 (TB)在模型制作前、后不同时相的变化 ,同时观察脏器转移、组织病理学改变、影像学表现及实验动物的自然生存时间。结果 接种后 3周均可见到肿瘤生长 ,肿瘤直径为 1.5～ 2 .0cm左右 ,肿瘤接种成功率为 10 0 % ;彩超检查显示肝脏有结节样增强回声 ,CT示肿瘤为低密度病灶 ,增强扫描见病灶不均匀强化。移植肿瘤外观呈灰白色 ,扪之质地较硬 ,无明显的包膜 ,剖开肿瘤中心可见灶性坏死 ;光镜下见肿瘤细胞核浆比例大 ,细胞排列紊乱 ,呈浸润性生长 ;动物接种后的自然生存时间为 4 0～ 5 3d ,死亡原因主要为肿瘤生长及转移引起的多脏器功能衰竭。结论 兔肝VX2 肿瘤从病理表现、病理过程及转移均与人肝癌相似 ,该模型具有容易复制、生长周期短、成功率高及模型稳定等特点 ,是一种较理想的肝肿瘤实验动物模型。
Objective To investigate the establishment of rabbit liver VX2 tumor model and provide experimental evidence for its application. Methods The VX2 cell line was used to replicate the rabbit liver tumor model and to improve its production method. The growth of the tumor was observed. ALT, AST and total bilirubin (TB) Before and after making changes in different phases, while observing organ metastasis, histopathological changes, imaging findings and experimental animals, the natural survival time. Results Three weeks after inoculation, tumor growth was observed. The tumor diameter was about 1.5-2.0 cm and the success rate of tumor inoculation was 100%. The color Doppler ultrasound examination showed nodular enhancement echo in the liver. CT showed the tumor was low density and enhanced See the uneven scanning enhancement. The appearance of grafted tumor was graying, the texture of the palpable mass was hard, and no obvious capsule was found. The tumor necrosis was seen in the center of the tumor. The proportion of nuclear cytoplasm of tumor cells was large and the cells arranged in disorder were infiltrative growth under light microscope. Natural survival time 40 ~ 5 3d, the main cause of death for tumor growth and metastasis caused by multiple organ failure. Conclusion The pathology, pathological process and metastasis of rabbit liver VX2 tumors are similar to that of human hepatocellular carcinoma. The model of rabbit liver VX2 tumor has the characteristics of easy replication, short growth cycle, high success rate and stable model. It is an ideal animal model of liver tumor.