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1941年,A.J.P.Martin和R.L.M.Synge使氨基酸的混合溶液通过填充了含一定水分的硅胶的吸附柱,再用氯仿冲洗,使得各氨基酸相互分离开来。稍后,在1944年,Martin和另外两个作者一起用滤纸代替硅胶,同样成功地使各氨基酸相互分离。他们把这种分离方法称为纸(分配)色谱法。 纸色谱法不仅有分离速度快,设备简单等优点,而且能对微克量的样品进行分离和鉴定;对难于用化学方法进行分离的元素,如稀土元素,铌和钽,锆和铪,硒和碲以及铂族元素等,在一定的条件下,也都可用纸色谱法进行有效地分离。因此,这个方法一出现,
In 1941, A.J.P. Martin and R.L.M.Synge mixed the amino acid solution through an adsorption column packed with a silica gel containing a certain amount of water and then washed with chloroform to separate the amino acids from each other. Later, in 1944, Martin and two other authors replaced the silica gel with filter paper, again succeeding in separating the amino acids from one another. They call this separation method paper (dispensing) chromatography. Paper chromatography not only has the advantages of fast separation and simple equipment, but also can separate and identify micrograms of samples. For the elements that are difficult to be separated by chemical methods, such as rare earth elements, niobium and tantalum, zirconium and hafnium, selenium and Tellurium and platinum group elements, under certain conditions, can also be used paper chromatography for effective separation. Therefore, this method appears,