论文部分内容阅读
目的研究饮食限制对高血脂小鼠抗氧化能力和Sirt1(沉默信息调节因子2相关酶1)的影响。方法建立高血脂小鼠模型,随机分高血脂对照组,限食30%组和限食50%组,同时设正常对照组,每组15只小鼠;正常对照组小鼠常规饲养,高血脂对照组小鼠自由摄食高脂饲料,限食30%组和限食50%组小鼠分别按照高血脂对照组平均摄食量的70%和50%饲喂高脂饲料;5 w后,取血液组织,解剖小鼠,取心、肝、脑等组织,测定其中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)等酶活性和总抗氧化能力(T-AOC)及丙二醛(MDA)含量;Western blotting检测肝脏和脂肪组织中Sirt1的表达。结果高血脂对照组小鼠心脏中SOD活性、血浆中CAT活性及心、肝、脑中的T-AOC均显著低于正常对照组(P<0.01或P<0.05),肝脏中MDA含量显著高于正常对照组(P<0.01),而限食30%组小鼠心脏中SOD、CAT活性,血浆中CAT活性,心、脑中T-AOC均显著高于高血脂对照组(P<0.01或P<0.05),MDA低于高血脂对照组;限食50%组小鼠心脏中SOD活性、血浆和肝脏中CAT活性亦均显著高于高血脂对照组(P<0.01);总体评价结果显示,两限食组小鼠SOD活性及限食30%组T-AOC均显著高于高血脂对照组(P<0.01);高血脂对照组小鼠肝和脂肪组织中Sirt1的表达量均降低,而两限食组较高血脂对照组显著升高(P<0.05或P<0.01)。结论饮食限制通过上调Sirt1的表达以增强高血脂小鼠的抗氧化能力。
Objective To investigate the effects of dietary restriction on antioxidant capacity and Sirt1 (Silencing Information Regulator-2-related enzyme 1) in hyperlipidemic mice. Methods A hyperlipidemia mouse model was established and randomly divided into hyperlipidemia control group, 30% restriction group and 50% restriction group. At the same time, a normal control group with 15 mice in each group was established. Normal control mice were fed routinely, The mice in the control group were fed with high-fat diet freely. The mice in the 30% and the 50% diets were fed with high-fat diet according to 70% and 50% of the average food intake of the hyperlipemia control group respectively. After 5 weeks, The activities of SOD, CAT, T-AOC and malondialdehyde (MDA) in tissues, tissues, heart, liver, (MDA). Western blotting was used to detect the expression of Sirt1 in liver and adipose tissue. Results The activities of SOD and CAT in plasma and T-AOC in heart, liver and brain of hyperlipidemic mice were significantly lower than those of normal control group (P <0.01 or P <0.05), and the content of MDA in liver was significantly higher (P <0.01). However, SOD and CAT activity, CAT activity in plasma and T-AOC in heart and brain were significantly higher in the 30% dieting mice than those in the hyperlipemia control group (P <0.01 or P <0.01) P <0.05), MDA was lower than that of hyperlipidemia control group. SOD activity in the heart of mice fed with the limited diet and CAT activity in the liver and liver were significantly higher than those in the hyperlipemia control group (P <0.01) (P <0.01). The expression of Sirt1 in the liver and adipose tissue of the hyperlipidemic mice decreased, while the SOD activity of the mice in the two groups limited diet and the T-AOC in the 30% The two diet group higher plasma lipid control group was significantly higher (P <0.05 or P <0.01). Conclusion Dietary restriction enhances the antioxidant capacity of hyperlipidemic mice by up-regulating Sirt1 expression.