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目的探讨建立刚地弓形虫稳定垂直传播小鼠模型的方法。方法将42只孕8dBALB/c小鼠随机分为(A-F组),正常对照A组(孕鼠经腹腔注射无菌PBS 0.2ml/只)和感染组(B~F)(每只小鼠经腹腔接种不同剂量的弓形虫速殖子10、20、40、80个/0.2ml)。观察并记录孕鼠的体重改变、行为反应、流产率与活胎率;孕18d时剖腹,取出胎鼠进行形态学观察。结果感染组小鼠出现倦怠、竖毛、弓背等异常反应,且随速殖子感染数量的增加异常反应程度逐渐严重;F组孕鼠死亡2只。感染组较对照组体重增加不明显,F组体重减轻;孕18d时D~F组体重与对照组比较差异有统计学意义(P<0.05)。感染组出现流产、死胎等,F组存活孕鼠腹中无胎鼠。D~F组流产率与对照组相比差异有统计学意义(P<0.05);B~F组活胎率与对照组比较差异有统计学意义(P<0.05)。E~F组胎鼠体重、体长、尾长与对照组比较差异有统计学意义(P<0.05)。结论成功建立刚地弓形虫垂直传播小鼠模型,BALB/c小鼠孕8d腹腔注射接种RH株弓形虫速殖子40个/(0.2ml·只)为适宜感染剂量。
Objective To explore a method of establishing a stable vertical transmission mouse model of Toxoplasma gondii. Methods Twenty-four pregnant 8 dB ALB / c mice were randomly divided into three groups: control group A, control group A (intraperitoneal injection of sterile PBS 0.2 ml / only) and infected group (B ~ F) Toxoplasma gondii tachyzoites were inoculated intraperitoneally with 10, 20, 40, 80 / 0.2 ml). The weight changes, behavioral responses, abortion rate and live fetus rate of pregnant rats were observed and recorded. Caesarean section was performed on the 18th day of gestation, and the fetus was removed for morphological observation. Results In the infected group, there was an abnormal reaction such as burnout, vertical hair and dorsum of the bow, and the degree of abnormal reaction gradually increased with the increase of the number of tachyzoites. In the F group, two pregnant mice died. The body weight of the infected group was not significantly increased compared with the control group, and the body weight of the infected group was reduced. The body weight of the group D ~ F at 18th day of gestation was significantly different from that of the control group (P <0.05). Infection group miscarriage, stillbirth and so on, F group surviving pregnant rats belly without fetal rats. There was significant difference in miscarriage rate between control group and control group (P <0.05). There was significant difference between control group and control group (P <0.05). The body weight, body length and tail length of E ~ F group were significantly different from the control group (P <0.05). Conclusion The Toxoplasma gondii vertical transmission mouse model was successfully established. The BALB / c mice were inoculated intraperitoneally with 40 Toxoplasma gondii RHM (0.2 ml·)