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目的利用同步热分析仪对14份不同地点收集但栽培环境相同的附子种质资源进行热分析研究,为附子种质资源鉴定提供新依据。方法 SDT Q600同步热分析仪对供试材料进行25~800℃程序升温扫描,得到其热分析曲线,根据TG-DTG-DSC曲线的差异峰对材料进行层次聚类分析。结果所有供试材料的TG-DTG-DSC热分析曲线总体趋势一致,其中DSC曲线330~490℃和近690℃的两个放热峰表现显著差异,这两个差异峰为附子种质资源鉴定提供了新的参考依据。对供试材料的差异峰进行聚类,将14份附子材料聚为4类,该结果表明当前药用附子种植市场的种质混乱。结论热分析法具有样品用量少,整体性强,图谱易分析的优点,为附子种质资源鉴定提供了高效便捷的新方法。
OBJECTIVE To provide a new basis for the identification of Aconitum germplasm resources by simultaneous thermal analysis of 14 aconite germplasm resources collected from different places but with the same cultivation environment. Methods SDT Q600 synchronous thermal analyzer was used to scan the materials at 25 ~ 800 ℃. The thermal analysis curves were obtained. The hierarchical clustering analysis was carried out according to the peak of TG-DTG-DSC curve. Results The TG-DTG-DSC thermal analysis curves of all the tested materials showed the same trend. The two exothermic peaks of DSC curves at 330 ~ 490 ℃ and near 690 ℃ showed significant differences, and the two peaks were identified as aconite germplasm resources Provided a new reference. Clustering the peaks of the test materials for differences and clustering 14 of the aconite materials into four categories indicates that the current medicinal aconite planting market is disrupted by germplasm. Conclusion The thermal analysis method has the advantages of less sample amount, more integrity and easier to analyze the spectrum, which provides a new efficient and convenient method for the identification of the aconite germplasm resources.