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目的 观察表皮生长因子受体 (EGFR)单克隆抗体是否可诱导人肺癌SPCA1细胞凋亡 ,以进一步说明EGFR单抗所具有的抗肿瘤作用。方法 以不同浓度的EGFR单克隆抗体干预培养人肺腺癌SPCA1细胞株 72h ,收集癌细胞并提取DNA ,采用凝胶电泳法和流式细胞仪分析细胞凋亡现象。结果 凝胶电泳显示 ,单抗浓度为 1 .0 μmol/L时 ,可见明显的凋亡梯状条带出现。流式细胞仪分析发现 ,在EGFR单抗干预培养的各SPCA1细胞样本存在低荧光的亚G1细胞群 ,而在空白对照和正常非相关IgG对照样本均未见低荧光细胞群存在。结论 表皮生长因子受体单克隆抗体可诱导人肺癌SPCA1细胞出现凋亡
Objective To observe whether epidermal growth factor receptor (EGFR) monoclonal antibody can induce apoptosis in human lung cancer SPCA1 cells to further illustrate the anti-tumor effect of EGFR monoclonal antibody. Methods Human lung adenocarcinoma cell line SPCA1 was cultured with different concentrations of EGFR monoclonal antibody for 72 hours. The cancer cells were collected and DNA was extracted. Cell apoptosis was analyzed by gel electrophoresis and flow cytometry. Results Gel electrophoresis showed that when the concentration of monoclonal antibody was 1.0 μmol/L, obvious ladders of apoptosis appeared. Flow cytometry analysis revealed that low-fluorescence sub-G1 cell populations were present in each SPCA1 cell sample cultured with EGFR mAb, whereas no low-fluorescence cell populations were present in both blank and normal non-associated IgG control samples. Conclusion Epidermal growth factor receptor monoclonal antibody can induce apoptosis in human lung cancer SPCA1 cells