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目的:建立RP-HPLC测定人体血浆中拉米夫定浓度。方法:采用ODS色谱柱(4．6 mm×150 mm,5μm),以乙腈: 40 mmol·L-1的KH2PO4缓冲液(含0．1％三乙胺,pH 3．5)=18:82为流动相,法莫替丁为内标;流速:1．0 ml·min-1;紫外检测波长为270 nm。结果:拉米夫定在2-2 500 ng·ml-1。浓度范围内线性关系良好(r=0．997 3),最低检测限为0．5 ng·ml-1,平均绝对回收率为91．6％(3．2％,n=15),日内与日间RSD均小于5％。结论:本法操作简单,灵敏度高,可以用于拉米夫定的药动学研究及血药浓度监测。
Objective: To establish a RP-HPLC for the determination of lamivudine in human plasma. METHODS: ODS column (4.6 mm × 150 mm, 5 μm) was used with acetonitrile (40 mmol·L -1) in KH 2 PO 4 buffer (containing 0.1% triethylamine and pH 3.5) As mobile phase, famotidine as internal standard; flow rate: 1.0 ml · min-1; UV detection wavelength was 270 nm. Results: Lamivudine ranged from 2-2 500 ng · ml-1. The linear range was good (r = 0.997 3), the lowest detection limit was 0.5 ng · ml-1, the average absolute recovery was 91.6% (3.2%, n = 15) Daytime RSDs were less than 5%. Conclusion: The method is simple, sensitive and can be used for lamivudine pharmacokinetics and blood concentration monitoring.