论文部分内容阅读
为了探讨8号染色体三体(8三体)对急性粒单、单核细胞白血病(M4、M5)细胞生物学及临床特征的影响,应用G显带或R显带技术及流式细胞仪对56例M4、M5患者进行核型及免疫表型检测,并对其临床特征进行回顾性分析。结果表明:56例患者中34例(60.7%)正常核型;10例(17.9%)8三体异常核型,其中3例(5.4%)为单纯8三体核型,余下12例(21.4%)为其他异常核型(不包括8三体)。8三体患者的年龄偏大,外周血原始+幼稚细胞百分比和外周血白细胞(WBC)计数均较低,无病生存期(DFS)较短(P均<0.05)。8三体患者CD34、CD117和CD56的表达频率较高,而CD11b、CD14和CD15则较低(P分别<0.01、0.05、0.05、0.01、0.05和0.005)。结论:8三体的M4、M5患者预后较差,8三体可能与单核细胞分化成熟受抑有关。
In order to investigate the influence of chromosome 8 trisomy 8 on the biological and clinical features of acute myelomonocytic leukemia (M4, M5) cells, G-banding or R-banding technique and flow cytometry 56 cases of M4, M5 patients for karyotype and immunophenotype detection, and its clinical features were retrospectively analyzed. The results showed that 34 cases (60.7%) had karyotypes in 56 patients and 10 (17.9%) 8 trisomy in 3 cases (5.4%), while the remaining 12 cases (21.4% %) For other abnormal karyotype (excluding trisomy 8). 8 trisomy patients were older, the percentage of primal + naive cells in peripheral blood and peripheral white blood cells (WBC) were lower, and the disease-free survival (DFS) was shorter (all P <0.05). 8 trisomy patients had higher frequency of CD34, CD117 and CD56 expression, while CD11b, CD14 and CD15 were lower (P <0.01, 0.05, 0.05, 0.01, 0.05 and 0.005, respectively). Conclusion: The trisomy 8, M4, M5 patients with poor prognosis, trisomy 8 may be related to monocyte differentiation and maturation.